We are grateful for the kind gift of the CGTase enzyme isolated from Bacillus macerans provided by Amano Enzyme Inc., Nagoya, Japan. We thank the Villum Foundation and Carlsberg Foundation for financial support. 7KLV (OHFWURQLF6XSSOHPHQWDU\0DWHULDO (6, IRU&KHP&RPP MRXUQDOLV 7KH 5R\DO 6RFLHW\RI&KHPLVWU\

15 Dec 2014 CGTase was purchased from Amano Enzyme Europe Ltd (Milton Keynes, UK) and Toruzyme 3.0L was obtained from Novozyme A/S (Bagsvaerd,

the Synthesis of β-maltosides of resveratrol. CGTase (Paenibacillus macerans) was purchased from Amano Enzyme Inc. Resveratrol 3-maltoside (8) and resveratrol 4′-β-maltoside (9) were produced individually as fol-lows. To a solution containing resveratrol 3-β-glucoside (2) or resveratrol 4′-β-glucoside (3) (0.1mmol) and (Toruzyme 3.0L) worked better than that from Bacillus macerans (CGTase Amano). The transglycosylation activity of CGTase is well reported, as it is able to glucosylate other phenolic compounds such The enzyme from Thermoanaerobacter sp. (Toruzyme 3.0L) worked better than that from Bacillus macerans (CGTase Amano). The transglycosylation activity of CGTase is well reported, as it is able to glucosylate other phenolic compounds such as resveratrol [ 26, 27 ], catechin [ 28 ], hydroquinone [ 29 ], kaempferol [ 30] or genistein [ 31 ]. Table 1.

Cgtase amano

These enzymes have been Amano Enzyme was founded 120 years ago in Japan as a pharmaceutical business, expanding into specialty enzymes in 1948 with our first item—malt diastase. Today, we have grown into one of the top enzyme manufacturers, producing enzyme solutions for any industry and every need. Synthesis of β-maltosides of resveratrol. CGTase (Paenibacillus macerans) was purchased from Amano Enzyme Inc. Resveratrol 3-maltoside (8) and resveratrol 4′-β-maltoside (9) were produced individually as fol-lows. To a solution containing resveratrol 3-β-glucoside (2) … In our work, the CGTase from Thermoanaerobacter sp. (Tor- uzyme 3.0 L) gave a higher yield than that from Bacil- lus macerans (CGTase Amano). In contrast with the CGTase from Bacillus macerans (the HPLC chroma- togram showed four different compounds with in- creasing concentration, indicating the formation of the so-called analogous series (A) reaction of DGAS, (B) reaction of CGTase (Amano Co.).

CGTase derived from Bacillus macerans was obtained as a kind gift from Amano Enzyme, Inc., Nagoya, Japan, in a stock solution that was used as supplied and stored at 5 °C.

회사명 : 바이오시스 / 대표 : 박동주 / 주소 : 부산광역시 해운대구 달맞이길62번길 3 (한신빌딩 4층) / tel : 051-746-7605 / fax : 051-746-7607

ATCC 53627 CGTase (Toruzyme 3.0L,) from Novozymes (Bagsvaerd, Denmark). These enzymes have been Amano Enzyme was founded 120 years ago in Japan as a pharmaceutical business, expanding into specialty enzymes in 1948 with our first item—malt diastase. Today, we have grown into one of the top enzyme manufacturers, producing enzyme solutions for any industry and every need.

Amano Enzyme was founded 120 years ago in Japan as a pharmaceutical business, expanding into specialty enzymes in 1948 with our first item—malt diastase. Today, we have grown into one of the top enzyme manufacturers, producing enzyme solutions for any industry and every need.

In contrast with the CGTase from Bacillus macerans (the HPLC chroma- togram showed four different compounds with in- creasing concentration, indicating the formation of the so-called analogous series (A) reaction of DGAS, (B) reaction of CGTase (Amano Co.). Mass spectra were obtained by Single Ion Recording (SIR) in positive mode. The fragment ions were appeared owing to the analysis condition of HPLC/QDa, accordingly, DA3 (m/z+ 741) showed its fragment (m/z+ 417) by reduction of molecular weight of two glucosyl (MW 162 × 2) moieties. Amano Enzyme, Inc. (Japan), and was used without further puriﬁ-cation.

All other chemicals used were of reagent grade.
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A novel enzymatic process for cyclodextrin (CD) production was developed by utilizing sucrose as raw material instead of corn starch. Cyclodextrin glucanotransferase (CGTase) from Bacillus macerans was applied to produce the CDs from linear α-(1,4)-glucans, which were obtained by Neisseria polysaccharea amylosucrase (NpAS) treatment on sucrose.

Ciclomaltodextrin. Glucanotransferasa. (CGTase).
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Cyclodextrin glucanotransferase (CGTase) from Bacillus macerans was applied to produce the CDs from linear α- (1,4)-glucans, which were obtained by Neisseria polysaccharea amylosucrase (Np AS) treatment on sucrose. The greatest CD yield (21.1%, w/w) was achieved from a one-pot dual enzyme reaction at 40 °C for 24 h.

In contrast with the CGTase from Bacillus macerans (the HPLC chromatogram showed four different compounds with Enzymatic glycosylation of curcumin 4‘-O-β-D-glucopyranoside (2) with CGTase afforded curcumin 4‘-O-β-glucooligosaccharides . Soluble starch was used as a glucose-donor.

Amano Enzyme was founded 120 years ago in Japan as a pharmaceutical business, expanding into specialty enzymes in 1948 with our first item—malt diastase. Today, we have grown into one of the top enzyme manufacturers, producing enzyme solutions for any industry and every need.

Quercetin and its glycoside products were separated on reverse phase columns using acetonitrile/water. Mass spectra were obtained Munich, Germany. CGTase derived from Bacillus macerans was obtained as a kind gift from Amano Enzyme, Inc., Nagoya, Japan, in a stock solution that was used as supplied and stored at 5 °C.

2009a). Cyclodextrin glucanotransferase from Bacillus macerans (CGTase Amano) was purchased from Amano Pharmaceutical Co., Ltd. (Nagoya, Japan).